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e gel 1 kb plus dna ladder  (Thermo Fisher)


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    Structured Review

    Thermo Fisher e gel 1 kb plus dna ladder
    E Gel 1 Kb Plus Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+gel+1+kb+plus+dna+ladder/1KB+PLUS+DNA+LADDER/pm41569017-80-17-23
    Average 96 stars, based on 1 article reviews
    e gel 1 kb plus dna ladder - by Bioz Stars, 2026-09
    96/100 stars

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    Amplification:

    Article Title: Single-cell measurement of higher-order 3D genome organization with scSPRITE
    Article Snippet: Each of the tubes were filled to 30 μl using 15 μl of Q5 Hot Start High-Fidelity 2× Master Mix (NEB, no. M0493S), 1.5 μl of 20× EvaGreen (Biotium, no. 31000-T), 1.2 μl of 25 μM indexed Illumina primers and 0.3 μl of H 2 O. Real-time PCR amplification proceeded for 14 cycles, which was when the libraries entered exponential amplification but had not plateaued. .. After amplification, each of the libraries was diluted four-fold before running on a 1% agarose E-gel (Life Technologies, no. G402001) with an E-Gel 1-Kb Plus DNA Ladder (Life Technologies, no. 10488090) as a reference. ..

    Article Title: Advancing Trypanosoma cruzi N-myristoyltransferase as a drug target for Chagas disease through in silico discovery and biochemical evaluation
    Article Snippet: .. Genomic DNA was isolated from CL Brener epimastigotes, and the 1.3 kb TcNMT ORF was amplified using Pfu DNA polymerase (Promega Corporation, Madison, WI, United States) under the following conditions: initial denaturation at 95 °C for 2 min; followed by 30 cycles of denaturation at 95 °C for 30 s, annealing at 58 °C for 30 s, and extension at 72 °C for 1.5 min; with a final extension at 72 °C for 10 min. PCR and digestion products were analyzed by agarose gel electrophoresis alongside the appropriate DNA size markers, as follows: 100 bp DNA Ladder (Invitrogen, Cat. No. 15628019) for , E-Gel 1 Kb Plus DNA Ladder (Invitrogen, Cat. No. 10488090) for , E-Gel 1 Kb Plus Express DNA Ladder (Invitrogen, Cat. No. 10488091) for , and GeneRuler 1 Kb DNA Ladder (Thermo Fisher Scientific, Cat. No. SM0312) for . .. The PCR product was purified, ligated into the Zero Blunt TOPO vector (Invitrogen (Thermo Fisher Scientific), Carlsbad, CA, United States), and the insert was verified by Sanger sequencing.

    Marker:

    Article Title: A plasmid containing the human metallothionein-II gene selectively distinguishes trivalent lanthanum from several divalent heavy metal cations during monoclonal antibody-assisted agarose gel electrophoresis.
    Article Snippet: Trivalent lanthanide ions are known for their ability to interact with calcium-binding sites in various proteins.. There is a need to assess the bioavailability of lanthanides and other heavy metals introduced into the body as components of implants or as contrast agents.. This study aimed to develop a method to address bioavailability and/or presence of trivalent lanthanide ions by examining electrophoretic mobility in an agarose gel of a plasmid harboring the human metallothionein-II gene (hMT-II).

    Isolation:

    Article Title: Advancing Trypanosoma cruzi N-myristoyltransferase as a drug target for Chagas disease through in silico discovery and biochemical evaluation
    Article Snippet: .. Genomic DNA was isolated from CL Brener epimastigotes, and the 1.3 kb TcNMT ORF was amplified using Pfu DNA polymerase (Promega Corporation, Madison, WI, United States) under the following conditions: initial denaturation at 95 °C for 2 min; followed by 30 cycles of denaturation at 95 °C for 30 s, annealing at 58 °C for 30 s, and extension at 72 °C for 1.5 min; with a final extension at 72 °C for 10 min. PCR and digestion products were analyzed by agarose gel electrophoresis alongside the appropriate DNA size markers, as follows: 100 bp DNA Ladder (Invitrogen, Cat. No. 15628019) for , E-Gel 1 Kb Plus DNA Ladder (Invitrogen, Cat. No. 10488090) for , E-Gel 1 Kb Plus Express DNA Ladder (Invitrogen, Cat. No. 10488091) for , and GeneRuler 1 Kb DNA Ladder (Thermo Fisher Scientific, Cat. No. SM0312) for . .. The PCR product was purified, ligated into the Zero Blunt TOPO vector (Invitrogen (Thermo Fisher Scientific), Carlsbad, CA, United States), and the insert was verified by Sanger sequencing.

    Polymerase Chain Reaction:

    Article Title: Advancing Trypanosoma cruzi N-myristoyltransferase as a drug target for Chagas disease through in silico discovery and biochemical evaluation
    Article Snippet: .. Genomic DNA was isolated from CL Brener epimastigotes, and the 1.3 kb TcNMT ORF was amplified using Pfu DNA polymerase (Promega Corporation, Madison, WI, United States) under the following conditions: initial denaturation at 95 °C for 2 min; followed by 30 cycles of denaturation at 95 °C for 30 s, annealing at 58 °C for 30 s, and extension at 72 °C for 1.5 min; with a final extension at 72 °C for 10 min. PCR and digestion products were analyzed by agarose gel electrophoresis alongside the appropriate DNA size markers, as follows: 100 bp DNA Ladder (Invitrogen, Cat. No. 15628019) for , E-Gel 1 Kb Plus DNA Ladder (Invitrogen, Cat. No. 10488090) for , E-Gel 1 Kb Plus Express DNA Ladder (Invitrogen, Cat. No. 10488091) for , and GeneRuler 1 Kb DNA Ladder (Thermo Fisher Scientific, Cat. No. SM0312) for . .. The PCR product was purified, ligated into the Zero Blunt TOPO vector (Invitrogen (Thermo Fisher Scientific), Carlsbad, CA, United States), and the insert was verified by Sanger sequencing.

    Agarose Gel Electrophoresis:

    Article Title: Advancing Trypanosoma cruzi N-myristoyltransferase as a drug target for Chagas disease through in silico discovery and biochemical evaluation
    Article Snippet: .. Genomic DNA was isolated from CL Brener epimastigotes, and the 1.3 kb TcNMT ORF was amplified using Pfu DNA polymerase (Promega Corporation, Madison, WI, United States) under the following conditions: initial denaturation at 95 °C for 2 min; followed by 30 cycles of denaturation at 95 °C for 30 s, annealing at 58 °C for 30 s, and extension at 72 °C for 1.5 min; with a final extension at 72 °C for 10 min. PCR and digestion products were analyzed by agarose gel electrophoresis alongside the appropriate DNA size markers, as follows: 100 bp DNA Ladder (Invitrogen, Cat. No. 15628019) for , E-Gel 1 Kb Plus DNA Ladder (Invitrogen, Cat. No. 10488090) for , E-Gel 1 Kb Plus Express DNA Ladder (Invitrogen, Cat. No. 10488091) for , and GeneRuler 1 Kb DNA Ladder (Thermo Fisher Scientific, Cat. No. SM0312) for . .. The PCR product was purified, ligated into the Zero Blunt TOPO vector (Invitrogen (Thermo Fisher Scientific), Carlsbad, CA, United States), and the insert was verified by Sanger sequencing.

    Nucleic Acid Electrophoresis:

    Article Title: Environmental Monitoring for Listeria Species in Controlled Atmosphere Apple Storage Facilities.
    Article Snippet: 147 Amplification of a 780 bp fragment of sigB was carried out on extracted DNA via hot start PCR 148 using previously described methods (Cai 2002) L. monocytogenes and L. innocua served as 149 positive controls while qPCR Grade Water (Fisher Scientific, Hampton, NH) served as the 150 negative control. .. Amplicons were visualized by gel electrophoresis using 1% E-Gel Double 151 Comb (Invitrogen, Waltham, MA) with the E-Gel 1 kb Plus DNA Ladder (Invitrogen, Waltham, 152 MA). ..



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